Laser-Induced Acoustic Desorption of Natural and Functionalized Biochromophores

Author(s): U. Sezer, L. Wörner, J. Horak, L. Felix, J. Tüxen, C. Götz, A. Vaziri, M. Mayor, M. Arndt

Journal: analytical chemistry

Volume: 87

Page(s): 5614-5619

Year: 2015

DOI Number: DOI: 10.1021/acs.analchem.5b00601

Link: Link to publication

Abstract:

Laser-induced acoustic desorption (LIAD) has recently been established as a tool for analytical chemistry. It is capable of launching intact, neutral, or low charged molecules into a high vacuum environment. This makes it ideally suited to mass spectrometry. LIAD can be used with fragile biomolecules and very massive compounds alike. Here, we apply LIAD time-of-flight mass spectrometry (TOF-MS) to the natural biochromophores chlorophyll, hemin, bilirubin, and biliverdin and to high mass fluoroalkyl-functionalized porphyrins. We characterize the variation in the molecular fragmentation patterns as a function of the desorption and the VUV postionization laser intensity. We find that LIAD can produce molecular beams an order of magnitude slower than matrix-assisted laser desorption (MALD), although this depends on the substrate material. Using titanium foils we observe a most probable velocity of 20 m/s for functionalized molecules with a mass m = 10 000 Da.

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